HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Thomas, M. Articles by Le Bouvier, G. Search for Related Content PubMed PubMed Citation Articles by Thomas, M. Articles by Le Bouvier, G. Agricola Articles by Thomas, M. Articles by Le Bouvier, G.

Transformation of Bovine Cell Cultures by Preparations of Polyoma Virus

G. Le Bouvier

M.R.C. Experimental Virus Research Unit, Institute of Virology, University of Glasgow, Glasgow, Scotland

Transformation, i.e. heritable morphological and growth changes, of cultured bovine cells induced by bovine papilloma virus has been reported by several authors (1, 2). We report here the finding that suspensions containing polyoma virus which has the ability to cause a transformation in cultured cells of several species of rodents (3–6) can also cause proliferation and various persistent alterations in certain bovine tissue culture cells.

The cellular changes were first recognized in two clones of diploid cells, P-3 and P-6, obtained from cultures of foetal bovine skin (7), after exposure to two different preparations of plaque-purified polyoma virus (Toronto small-plaque strain). The cells were seeded in 50 mm. diameter plastic Petri dishes (2 x 10⁵ cells per dish) and were grown and maintained at 36° in a humidified atmosphere of 5% (v/v) CO₂ in air. The medium consisted of modified Eagle's medium containing additional amino acids and vitamins (8) supplemented with 10% (v/v) unheated foetal calf serum, penicillin (100 u./ml.) and streptomycin (100µg./ml.).

^* Laboratoire d'Hématologie expérimentale, Institut de recherches sur les leucémies, Hôpital Saint-Louis, Paris Xème, France.

Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Conn., U.S.A.

Received 31 August 1966; accepted 25 September 1966.