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Denaturation of Sindbis Virus RNA with Dimethyl Sulphoxide

Department of Microbiology Queen's University, Kingston, Ontario, Canada

Recent experiments have indicated that the mol.wt. of the RNA of group A arboviruses is 3.5 to 5 x 10⁶ (Cartwright & Burke, 1970; Strauss, 1969; Dobos & Faulkner, 1970). Denaturation of the 42s arbovirus genome by heat, dimethyl sulphoxide (DMSO) or 8 M-urea resulted in: (i) the reduction of sedimentation coefficient to 26s, (ii) loss of infectivity, (iii) increased electrophoretic mobility in polyacrylamide gels indicating a reduction in mol.wt. to approximately 1.7 x 10⁶ (Sreevalsan et al. 1968; Sprecher-Goldberger, 1967; Dobos & Faulkner, 1969; Cartwright & Burke, 1970; Dobos & Faulkner, 1970). It appears that the arbovirus genome is not a single continuous polynucleotide chain but may consist of fragments which upon denaturation separate into pieces of mol.wt. 1.7 x 10⁶ (Cartwright & Burke, 1970; Dobos & Faulkner, 1970).

^* Present address: Division of Biochemistry, National Institute for Medical Research, Mill Hill, London, N.W. 7.

Received 30 June 1970; accepted 12 October 1970.