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J Gen Virol 10 (1971), 107-110; DOI 10.1099/0022-1317-10-1-107
© 1971 Society for General Microbiology
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Rescue of Murine Sarcoma Virus Genome in Mixed Cultures of ‘Non-producer’ Hamster Tumour Cells and Helper Virus Carrier Cell Lines

S. S. Chang

Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland

R. V. Gilden

Flow Laboratories, Inc., Rockville, Maryland

R. J. Huebner

Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland

The defective genome of murine sarcoma virus (MSV)* (MOLONEY isolate) may be rescued from non-producer hamster tumour cells (HT-1) by co-cultivation of the latter and mouse embryo cells with added helper murine leukaemia viruses (MuLV) (Huebner et al. 1966; Huebner, 1967). Rescue of MSV from HT-1 cells by co-cultivation with MuLV-carrier cells has not, however, been described. This report describes the retrieval of MSV from mixed cultures of HT-1 and various MuLV-carrier cells of mouse and hamster origin. Moreover, it was found that the frequency of success and titres of MSV recovered by co-cultivation of HT-1 and F4, one of the MuLV-carrier mouse cell lines, were much higher than those obtained by the conventional method. We have analysed the factors responsible for such high efficiency of genome rescue.

Among the MuLV-carrier cell lines used, F4 and F5 were derived from two of nine Rauscher pseudotype MSV (MSV(RLV) transformed foci of NIH Swiss mouse embryo cells isolated by trypsinization within a cloning ring.

* Since MOLONEY strain murine sarcoma virus is the subject of the present study, the abbreviation MSV will be used in this article to denote this virus unless otherwise specified.

Received 3 September 1970; accepted 14 October 1970.




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Copyright © 1971 by the Society for General Microbiology.