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J Gen Virol 10 (1971), 29-36; DOI 10.1099/0022-1317-10-1-29
© 1971 Society for General Microbiology
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Studies of the Enhancement of an Adenovirus-associated Virus by Herpes Simplex Virus

N. R. Blacklow, R. Dolin and M. D. Hoggan

Laboratory of Infectious Diseases and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, U.S.A.

The defective adenovirus-associated viruses produce infectious progeny only in cells co-infected with an unrelated helper adenovirus. However, the adenovirus-associated viruses produce antigen detectable by immunofluorescence but not infectious virus in cells co-infected with herpes simplex virus. This incomplete helper effect provided by herpes simplex virus was studied in Hep-2 cells by quantitative kinetic procedures. Dose response studies showed that a single infectious adenovirus associated virus-1 particle and a single infectious herpes simplex virus particle were sufficient to initiate adenovirus-associated virus antigen synthesis. Adenovirus-associated virus-1 antigen was formed 6 hr after infection with both viruses, and at a time before the production of infectious herpes simplex virus. Co-infected cells, deprived of arginine, formed adenovirus-associated virus-1 antigen while undergoing an abortive herpes simplex virus infection in which infectious herpes simplex virus was not produced.

Sequential inoculation studies showed that the 6 hr latent period for adenovirus-associated virus antigen formation was lengthened, rather than shortened, by pre-infection with herpes simplex virus. Cytosine arabinoside treatment of co-infected cells within 4 to 6 hr after infection prevented adenovirus-associated virus antigen formation.

Received 17 July 1970; accepted 27 August 1970.




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Copyright © 1971 by the Society for General Microbiology.