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Evidence for an RNA Replicative Intermediate in Cells Infected with Newcastle Disease Virus

W. S. Robinson

^* Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115
Department of Medicine, Stanford University, Palo Alto, California 94305

Cells infected with Newcastle disease virus contained significant amounts of base-paired RNA. Base pairing in this RNA was suggested by its resistance to ribonuclease and by the sharp thermal transition from ribonuclease resistance to sensitivity at 83° in 0.01 M-NaCl. The base-paired RNA sediments heterogeneously with a peak at 35 S.

Preferential labelling of the base-paired RNA after short periods of incubation with [³H]uridine suggests that it may serve as an intermediate in the synthesis of single-stranded virus-specific RNA. The kinetics of [³H]uridine incorporation into the various species of virus-specific RNA are discussed.

Received 24 July 1970; accepted 5 October 1970.