HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, C. Articles by Choppin, P. W. Search for Related Content PubMed PubMed Citation Articles by Chen, C. Articles by Choppin, P. W. Agricola Articles by Chen, C. Articles by Choppin, P. W.

Parainfluenza Virus Surface Projections: Glycoproteins with Haemagglutinin and Neuraminidase Activities

The Rockefeller University, New York, New York 10021 U.S.A.

The parainfluenza virus SV5 has been found to contain six proteins with mol. wts ranging from about 41,000 to 76,000 (Caliguiri, Klenk & Choppin, 1969; Mountcastle, Compans & Choppin, 1971). The nucleocapsid of the virus particle is composed of a single polypeptide chain with a mol. wt. of about 61,000 (Mountcastle et al. 1970); the other five proteins are presumably associated with the virus envelope. Two of the proteins are covalently linked to carbohydrate (Klenk, Caliguiri & Choppin, 1970). We report here that these glycoproteins comprise the spikes or projections on the surface of the SV5 virus particle.

The w3 strain of SV5 (Choppin, 1964) was grown in MDBK cells (Choppin, 1969) in reinforced Eagle's medium (Bablanian, Eggers & Tamm, 1965) with 2% calf serum. Cells were inoculated at a multiplicity of about 10 p.f.u./cell.

Received 16 December 1970; accepted 28 December 1970.