HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Breindl, M. Search for Related Content PubMed PubMed Citation Articles by Breindl, M. Agricola Articles by Breindl, M.

The Structure of Heated Poliovirus Particles

Institut für Mikrobiologie, Medizinische Hochschule Hannover, Hannover, W. Germany and Roche Institute of Molecular Biology, Nutley, New Jersey, U.S.A.

Purified poliovirus preparations were heated and analysed by sucrose gradient centrifugation. They consisted of virus-like particles containing RNA and sedimenting at about 80s, empty 80s capsids, 35s virus RNA, and a non-sedimentable capsid polypeptide (VP 4). By electron microscopy the 80s ribonucleoprotein particles (80s RNP) were similar in appearance to intact poliovirus particles. They contained infectious, RNase-sensitive RNA that could be liberated from the capsid by treatment at room temperature with 1% sodium dodecyl sulphate. One of the virus polypeptides was missing, and they had lost the antigenicity of the mature virus and the ability to adsorb to HeLa cells.

Degradation of poliovirus particles probably occurs in two steps: the first is the splitting off of a minor part of the capsid protein (VP 4) followed under certain conditions by a liberation of the RNA from the capsid. The alteration of the physical and biological properties of the virus particle is probably due to the loss of the protein rather than to the liberation of the RNA.

Received 5 October 1970; accepted 21 January 1971.

This article has been cited by other articles:

				B. Gassilloud, L. Schwartzbrod, and C. Gantzer Presence of Viral Genomes in Mineral Water: a Sufficient Condition To Assume Infectious Risk? Appl. Envir. Microbiol., July 1, 2003; 69(7): 3965 - 3969. [Abstract] [Full Text] [PDF]

				S. Nuanualsuwan and D. O. Cliver Infectivity of RNA from Inactivated Poliovirus Appl. Envir. Microbiol., March 1, 2003; 69(3): 1629 - 1632. [Abstract] [Full Text] [PDF]

				S. Nuanualsuwan and D. O. Cliver Capsid Functions of Inactivated Human Picornaviruses and Feline Calicivirus Appl. Envir. Microbiol., January 1, 2003; 69(1): 350 - 357. [Abstract] [Full Text] [PDF]

				T. S. Baker, N. H. Olson, and S. D. Fuller Adding the Third Dimension to Virus Life Cycles: Three-Dimensional Reconstruction of Icosahedral Viruses from Cryo-Electron Micrographs Microbiol. Mol. Biol. Rev., December 1, 1999; 63(4): 862 - 922. [Abstract] [Full Text] [PDF]

				R. Basavappa, A. Gomez-Yafal, and J. M. Hogle The Poliovirus Empty Capsid Specifically Recognizes the Poliovirus Receptor and Undergoes Some, but Not All, of the Transitions Associated with Cell Entry J. Virol., September 1, 1998; 72(9): 7551 - 7556. [Abstract] [Full Text] [PDF]