J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 12 (1971), 239-247; DOI 10.1099/0022-1317-12-3-239
© 1971 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iinuma, M.
Right arrow Articles by Matsumoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iinuma, M.
Right arrow Articles by Matsumoto, T.
Agricola
Right arrow Articles by Iinuma, M.
Right arrow Articles by Matsumoto, T.

Studies on the Assembly of Newcastle Disease Virus: Incorporation of Structural Proteins into Virus Particles

M. Iinuma, Y. Nagai, K. Maeno, T. Yoshida and T. Matsumoto

Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya, Japan

The mechanism of assembly of Newcastle disease virus in chick embryo cells was investigated in two series of experiments. When protein synthesis was inhibited by addition of puromycin, cycloheximide or fluorophenylalanine at any time during the course of infection, subsequent virus production was soon inhibited. These drugs were inhibitory even when they were added to the cultures as late as 10 hr after infection, when large amounts of virus precursor proteins were present within the cell. In the second series of experiments, the kinetics and efficiency of incorporation of radioactive amino acid into virus particles were examined by the pulse-labelling technique. A 30 min. labelling period at the 3rd or 6th hr of infection resulted in the release of highly radioactive virus during the period of 11/2 hr immediately after the pulse. However, when pulse-labelling was performed at the 9th hr, the maximally labelled virus was found in the yield obtained 3 hr after the pulse, and the specific radioactivity of virus was less than 1% of that of the virus harvested from cultures labelled earlier. On the basis of these findings, possible mechanics of virus assembly are discussed.

Received 10 September 1970; accepted 21 April 1971.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1971 by the Society for General Microbiology.