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Purification of Oat Necrotic Mottle Virus with Silver Nitrate as Clarifying Agent^*

Canada Department of Agriculture, Research Station, Winnipeg 19, Manitoba, Canada

A satisfactory purification procedure for oat necrotic mottle virus involved clarification of the plant juice by silver nitrate, followed by two cycles of differential centrifugation, and then rate-zonal density gradient centrifugation. The concentrate was finally passed through a column of agarose gel. Antiserum to clarified sap from virus-free oats did not react with the final preparation. A medium consisting of 0.1 M-sodium citrate buffer, pH 6.2, containing 0.5 M-urea, was suitable for the concentrated virus. The virus particles were filamentous with a diameter of 11 nm. and a normal length of 720 nm.

^* Contribution No. 448, Canada Department of Agriculture, Research Station.

Received 23 October 1970; accepted 23 April 1971.