HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gazzolo, L. Articles by Martin-Berthelon, M. C. Search for Related Content PubMed PubMed Citation Articles by Gazzolo, L. Articles by Martin-Berthelon, M. C. Agricola Articles by Gazzolo, L. Articles by Martin-Berthelon, M. C.

The Presence of C-type RNA Virus Particles in a Rat Embryo Cell Line Spontaneously Transformed in Tissue Culture

D. imkovi

Unité de Virologie, I.N.S.E.R.M., Unit of Biological Carcinogenesis, International Agency for Research on Cancer, Lyon, France. Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia

Two established rat cell lines, WERC and TWERC, were studied for the presence of C-type RNA virus particles. WERC cells, spontaneously transformed in vitro, continuously released C-type RNA virus (WERC virus) by budding from the plasma membrane. Some of these virus particles (diameter about 100 nm.) showed an electron-lucent nucleoid of diameter 75 to 80 nm. and represented immature C-type virus. Others showed dense nucleoids of about 65 to 70 nm. diameter and looked like mature forms of murine leukaemia virus. Each cell showed 60 to 100 virus particles. The buoyant density of the virus particles in sucrose gradients was that expected for avian or murine RNA virus particles (1.16 g./ml.).

No virus particles were observed in the subline of cells (TWERC) originating from the malignant transformation with avian sarcoma virus (the PRAGUE strain of Rous sarcoma virus) of parental WERC cells.

^* Recipient of the Fellowship of the Ligue Nationale contre le Cancer.

Received 2 November 1970; accepted 4 May 1971.

This article has been cited by other articles:

				V. Specke, S. J. Tacke, K. Boller, J. Schwendemann, and J. Denner Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal models J. Gen. Virol., April 1, 2001; 82(4): 837 - 844. [Abstract] [Full Text]

				M. M. Lieber, R. E. Benveniste, D. M. Livingston, and G. J. Todaro Mammalian Cells in Culture Frequently Release Type C Viruses Science, October 5, 1973; 182(4107): 56 - 59. [Abstract] [PDF]