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J Gen Virol 12 (1971), 325-329; DOI 10.1099/0022-1317-12-3-325
© 1971 Society for General Microbiology
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Disc Electrophoretic Separation of Elongated Plant Viruses in Polyacrylamide-agarose Gels

G. Wolf

Universität Göttingen Institut für Phytopathologie Nikolausberger Weg 5a Göttingen, W. Germany

R. Casper

Biologische Bundesanstalt Institut für Virusserologie, Messeweg 11/12 Braunschweig, W. Germany

Disc electrophoresis in polyacrylamide gels has often been used for investigation of virus proteins (McCarthy, 1968; Sarkar & Schilde-Rentschler, 1968; Semancik, 1966), but has seldom been used to separate whole viruses (Semancik, 1966; Tiselius, Hjerten & Jerstedt, 1965). Tiselius et al. (1965) were the first to fractionate a plant virus, turnip yellow mosaic virus (TYMV) by electrophoresis in polyacrylamide gels. Semancik (1966) and Niblett & Semancik (1969, 1970) used this method for fractionation and characterization of the components of purified isometric plant viruses, but separation in polyacrylamide gel was not possible for viruses with elongated particles, such as tobacco mosaic virus (TMV) or tobacco rattle virus.

In this paper we report the electrophoresis of viruses with elongated particles in polyacrylamide-agarose gels of low polyacrylamide concentration. This method has been used before to fractionate nucleic acids and ribosomes (Peacock & Dingman, 1968; Dahlberg, Dingman & Peacock, 1969).

Received 17 March 1971; accepted 9 June 1971.




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