HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Turner, R. H. Search for Related Content PubMed Articles by Turner, R. H. Agricola Articles by Turner, R. H.

Electron Microscopy of Cells Infected with Narcissus Mosaic Virus

Rothamsted Experimental Station, Harpenden, Hertfordshire

The particles of narcissus mosaic virus (NMV) are rod-shaped with a modal length 548 to 568 nm. (Fig. 1), and morphologically similar to those placed in the potato virus X (PVX) group by Brandes (1964). However, its inclusion in this group is uncertain, because it seems unrelated serologically to any other virus in the group (Brunt, 1966). PVX produces some characteristic cytological effects, so that sections of leaves of Nicotiana clevelandii infected with NMV were examined in the electron microscope to see whether they resembled cells infected with PVX. They did not, and in contrast showed a strikingly different distribution of virus particles.

The sections were of systemically infected leaves, picked 3 weeks after the lower leaves of N. clevelandii plants had been mechanically inoculated with NMV. The pieces of leaf were fixed and embedded in Epon, as described by Milne (1970). Thin sections, mounted on uncoated grids, were stained in Reynolds' lead citrate for 1 min. and examined in a Siemens Elmiskop 1A operating at 60 kV.

Received 14 June 1971; accepted 28 June 1971.