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Electron Microscopic Study of Development of Vesicular Stomatitis Virus Using Ferritin-labelled Antibodies

Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University, New York, N.Y.

Significant virus autointerference was not observed in serial undiluted passages of vesicular stomatitis virus in Madin-Darby canine kidney cells. The ultra-structural features of vesicular stomatitis virus development into Madin-Darby canine kidney cells were studied by staining frozen-thawed cells with ferritin-conjugated antibody. The granular and fibrillar antigen could be readily identified in the cytoplasm within 4 hr after infection. Its appearance was followed closely by the differentiation of virus filaments at the cell surface. The virus antigen could be recognized in close proximity to virus particles; this material may represent virus nucleoprotein incorporated into budding virus particles.

Received 6 April 1971; accepted 13 May 1971.