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Macromolecule Synthesis in RTG-2 Cells Following Infection with Infectious Pancreatic Necrosis (IPN) Virus

Department of Microbiology College of Life Sciences and Agriculture University of Maine Orono, Maine USA 04473

Infectious pancreatic necrosis (IPN) virus is the causal agent of a highly contagious and destructive disease of young hatchery-reared trout (Wolf et al. 1960). Studies with metabolic inhibitors indicated that the virus nucleic acid is RNA (Malsberger & Cerini, 1965) and electron microscopy showed that the virus particles had a diameter of approximately 65 nm. and resembled those of reovirus (Moss & Gravell, 1969; Lightner & Post, 1969). This paper presents the results of a study of macromolecular synthesis in rainbow trout gonad (RTG) cells infected with IPN virus.

The continuous rainbow trout gonad cell line (RTG-2) (Wolf & Quimby, 1962) was propagated at 22° in growth medium (GM) consisting of Eagle's minimum essential medium with Earle's balanced salt solution (EBSS) and supplemented with 10% foetal bovine serum. Maintenance medium (MM) was the same except for the serum concentration which was reduced to 2%. Both GM and MM contained 100 units of penicillin, 100 µg. of streptomycin and 25 units of mycostatin per ml.

Received 10 June 1971; accepted 19 August 1971.