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J Gen Virol 13 (1971), 441-447; DOI 10.1099/0022-1317-13-3-441
© 1971 Society for General Microbiology
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Localization of Complement-fixing Antigens in Cells: Epstein-Barr Virus-induced Membrane and Interior Cell Antigens

Ariel Hollinshead, O'Bong Lee and T. C. Alford

Laboratory for Virus and Cancer Research, The George Washington University Medical Centre, 2300 K Street, N.W., Washington, D.C. 20037 U.S.A.

A direct study of the complement-fixing reactivity of cell membranes, soluble components of cell membranes and cell interiors of lymphoblast cell lines showed that with highly concentrated levels of antigen, complement-fixing reactivity could be measured consistently between the cell interior of lymphoma (IM1), leukaemic (4265) and Burkitt lymphoma (P3) cultured cells and sera from patients with infectious mononucleosis or Burkitt lymphoma. A measurable complement-fixing reactive cell-membrane antigen appeared to be present in IM1 and 4265 cells. Complement-fixing activity was also recovered in a soluble fraction of 4265 cell membranes after sonication and separation by Sephadex gel filtration. Separation of the complement-fixing reactive component from HL-A antigens was achieved by gel electrophoresis.

Received 18 March 1971; accepted 26 July 1971.


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Copyright © 1971 by the Society for General Microbiology.