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A Search for Recombination between Temperature-Sensitive Mutants of Vesicular Stomatitis Virus

Manitoba Cancer Treatment and Research Foundation and Department of Medical Microbiology University of Manitoba, Winnipeg, Canada

Studies of genetic recombination have been of considerable value in elucidating the structure and function of bacterial viruses and have also been of value for certain animal viruses (e.g. see Fenner, 1970). Pringle (1970) has reported recombination between ts mutants of vesicular stomatitis virus (VSV) when mutants from different complementation groups were used in mixed infections. We have looked for recombination in our collection of VSV mutants (Holloway, Wong & Cormack, 1970), also, and have obtained the results presented in this paper.

L-cell and virus stocks were maintained and assayed using the methods previously described (Holloway et al. 1970). For the present experiments, cell monolayers were prepared by seeding each of a number of 2 oz. Brockway bottles with 2.5 x 10⁶ cells. For mixed infection 2.5 x 10⁷ p.f.u. of each mutant were added in a total volume of 0.5 ml, giving a total m.o.i. of 20.

Received 5 July 1971; accepted 20 August 1971.