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Dependence of the Activity of an Influenza Virus Neuraminidase Upon Ca²⁺

Department of Microbiology John Curtin School of Medical Research Australian National University, Canberra, A.C.T., Australia

Divalent cations are necessary for the activity of neuraminidases from certain sources. Burnet & Stone (1947) and Burnet (1948) reported that Ca²⁺ was necessary for the activity and stability of a crude preparation of neuraminidase from Vibrio cholerae and this was confirmed by Ada, French & Lind (1961) for the purified V. cholerae enzyme. Likewise Warren & Spearing (1960) reported that the activity of a mammalian neuraminidase was enhanced by Ca²⁺. However, the isolated neuraminidase of influenza virus showed no dependence upon added Ca²⁺ until the enzyme was subjected to more stringent desalting on a Sephadex G-200 column (Wilson & Rafelson, 1967); the activity of the enzymes (of A₀, A₂ and B types) then increased 6-fold when Ca²⁺ was added. Earlier, Edney (1949) had shown that the enzymic activity of crude preparations of influenza B/LEE was enhanced about 4-fold by the addition of Ca²⁺. In this paper two strains of influenza virus were studied.

^* Present address: Division of Biological Sciences, University of Warwick, Coventry.

Received 23 July 1971; accepted 31 August 1971.

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