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J Gen Virol 14 (1972), 103-106; DOI 10.1099/0022-1317-14-1-103
© 1972 Society for General Microbiology
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Binding of Concanavalin A to the Envelope of Two Murine RNA Tumour Viruses

Jero Calafat and Philomena C. Hageman

Departments of Electron Microscopy and Biology Antoni van Leeuwenhoekhuis The Netherlands Cancer Institute, 108 Sarphatistraat Amsterdam, The Netherlands

Concanavalin A (Con. A), a phytagglutinin derived from Canavalia ensiformis, binds specifically to several branched carbohydrates (Goldstein, Hollerman & Merrick, 1965), containing terminal non-reducing {alpha}-D-glucopyranosyl, {alpha}-D-mannopyranosyl or beta-D-fructofuranosyl residues (Goldstein, Hollerman & Smith, 1965; Goldstein & So, 1965).

Recently Bernhard & Avrameas (1971) developed a method to visualize the reaction of a cell surface with Con. A with the electron microscope. This gave us the opportunity to use Con. A to study the structure of the envelope of two RNA tumour viruses that mature at the cell membrane. B particles of the mouse mammary tumour virus (MTV-s) and C particles of a murine leukaemia virus (Rauscher) were used for this purpose.

The similarity of the Con. A-polysaccharide interaction to antibody-antigen precipitation is striking (So & Goldstein, 1967). We therefore tried to observe directly the interaction between the virus particles and Con. A after negative staining with phosphotungstic acid (PTA), as has been done with the virus-antibody complexes (Lafferty & Oertelis, 1963).

Received 11 August 1971; accepted 23 September 1971.




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Copyright © 1972 by the Society for General Microbiology.