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Virus Inclusions Formed by the PM₂ Mutant of TMV

Rothamsted Experimental Station, Harpenden Hertfordshire

Plants infected with the FLAVUM or the NI-118 strain of TMV contain electron-dense cytoplasmic X-bodies (Kolehmainen, Zech & Von Wettstein, 1965; Kassanis & Milne, 1971), whereas plants infected with the type strain do not. Strain NI-118 produces insoluble coat protein, especially in infected plants that are kept at temperatures above 30° (Jockusch, 1966; Kassanis & Bastow, 1971), and it is possible that the X-bodies contain this protein. We thought that information about the nature of the X-bodies might be obtained by comparing the behaviour of the two variants of PM₂ only one of which forms insoluble coat protein. PM₂ was originally obtained by treating type TMV with nitrous acid and produces a defective protein unable to coat its RNA (Siegel, Zaitlin & Sehgal, 1962). The original PM₂ has a soluble protein, but a variant, isolated at Rothamsted from it, from a single local lesion, produces insoluble coat protein (Kassanis & Bastow, 1971).

Received 8 October 1971; accepted 18 October 1971.