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Differential Release of Cellular Lactate Dehydrogenase During Replication of Adenovirus Types 5 and 12

Department of Microbiology University of New Hampshire, Durham, N.H. 03824, USA

A characteristic c.p.e. is observed during growth of human adenoviruses in established cell lines. When infected cell cultures are examined by cytochemical techniques, basophilic and Feulgen-positive intranuclear inclusions are prominent features (Boyer, Denny & Ginsberg, 1959). However, the development and form of the mature inclusion differs according to the antigenic type of the infecting virus. Microscopic examination of unstained monolayers reveals a distinctive rounding and aggregation of cells into grape-like clusters. Even though infected cells show considerable c.p.e. they do not lyse and progeny virus particles are not readily released (Ginsberg, 1958; Denny & Ginsberg, 1961).

Normally adenoviruses grow only in cell cultures derived from tissues of their natural hosts (Pereira et al. 1963). As adenoviruses isolated from apes grow in human cells (Rowe, Hartley & Huebner, 1958), it was of interest to investigate the replication of human adenoviruses in ape cells.

Received 16 August 1971; accepted 29 October 1971.