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29 Bacteriophage Structural Proteins

Departmento de Bioquímica y Química Facultad de Medicina, Universidad de Chile, Casilla, 6671 Santiago-4, Chile

The proteins from bacteriophage 29 have been dissociated with 1% SDS and 2% 2-mercaptoethanol and have been examined by SDS-acrylamide gel electrophoresis. Seven different polypeptides bands have been detected in the purified virus. One of the bands (b₁) is frequently detected in different positions suggesting that it represents an aggregate rather than a very large polypeptide. The molecular weights of the other polypeptide bands (b₂, b₃, b_3–4, b₄, b₅ and b₆) estimated by co-electrophoresis with marker proteins are 93,000; 75,000; 65,000; 53,000; 41,000 and 30,000. Protein bands b₂, b₄ and b₆ compose 80% of the bacteriophage. The six structural polypeptides described here could account for approximately 60% of the information contained in the virus DNA.

^* Present address: Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, England.

Received 20 September 1971; accepted 26 October 1971.