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Electron Microscopy of Replicative Form and Single-stranded RNA of Alfalfa Mosaic Virus

Laboratoire des Virus des Plantes, Université Louis Pasteur, 8, rue Goethe, Strasbourg, France

Double stranded RNAs obtained from plants infected with alfalfa mosaic virus have been studied by electron microscopy using the Kleinschmidt technique. Two characteristic lengths were obtained. A change of length was observed when the two strands were separated with dimethylsulphoxide. This corresponded to a change of configuration from the A form in double-stranded RNA to the B form in single-stranded RNA.

Using dimethylsulphoxide it was possible to visualize single-stranded RNA molecules as elongated filaments.

The electron microscopic results are compared with reported mol. wt values for the different RNAs present in alfalfa mosaic virus.

Received 29 December 1971; accepted 3 March 1972.