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J Gen Virol 16 (1972), 173-183; DOI 10.1099/0022-1317-16-2-173
© 1972 Society for General Microbiology
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Heterogeneity in the Phospholipid Content of Purified Rabies Virus (ERA Strain) Particles

Frantisek Sokol, H. F. Clark and Emese György

Wistar Institute of Anatomy and Biology, and the World Health Organization International Reference Center for Rabies at The Wistar Institute, Philadelphia, Pennsylvania 19104

Natale Tomassini

Division of Experimental Pathology, The Children's Hospital of Philadelphia and Department of Pediatrics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19146, U.S.A.

When rabies virus (ERA strain) was purified by precipitation with zinc acetate, Sephadex filtration, and centrifuging in a sucrose density gradient, usually about half of the virus particles spontaneously lost a portion of their envelope phospholipids. Because of differences in buoyant density, partially delipidized virus particles (about 1.16 g./cm.3) can be separated from intact virus (about 1.14 g./cm.3) by centrifuging in a sucrose density gradient. High egg passage virus (FLURY strain) purified by the same procedure, did not exhibit this type of heterogeneity. The release of phospholipids from ERA strain virus particles did not cause a marked decrease in the infectivity of the virus, but the morphology of the virus envelope changed from bullet- to bag-shaped. The protein (glycoprotein) composition and the RNA contents of intact and partially delipidized virus forms were similar.

Received 18 January 1972; accepted 7 April 1972.




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Copyright © 1972 by the Society for General Microbiology.