HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schild, G. C. Articles by Pereira, H. G. Search for Related Content PubMed PubMed Citation Articles by Schild, G. C. Articles by Pereira, H. G. Agricola Articles by Schild, G. C. Articles by Pereira, H. G.

A Quantitative, Single-Radial-Diffusion Test for Immunological Studies with Influenza Virus

WHO World Influenza Centre National Institute for Medical Research, Mill Hill, London NW7 1AA England

Antibodies for influenza haemagglutinin and neuraminidase are assayed routinely in haemagglutination-inhibition (Hirst, 1942) and neuraminidase-inhibition (Ada, Lind & Laver, 1963) tests which involve reaction systems of three components. More recently, influenza antibodies have been detected by gel immuno-double-diffusion tests with influenza viruses disrupted by detergents (Styk & Hanna, 1966; Schild & Pereira, 1969). Although the latter method has the advantage that it detects antibodies for each of the major antigens of the virus in a single test system (Schild et al. 1972a; Schild, 1972) the quantitation of antibody is not readily achieved. In the present report we describe a quantitative, single-radial-diffusion test which is a sensitive, rapid and convenient method for the estimation of antibodies to the haemagglutinin and neuraminidase antigens of the influenza virus. A unique feature of this test is that only one component, the antibody, diffuses during the test; the other component, the antigen in the form of intact influenza virus particles, does not diffuse.

Received 7 March 1972; accepted 10 May 1972.