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Fate of Virus DNA in the Abortive Infection of Human Lymphoid Cell Lines by Epstein-Barr Virus

Department of Tumor Biology Department of Chemistry II Karolinska Institute, 104 01 Stockholm Sweden

Human lymphoid cell lines have so far only been established from cells exposed to Epstein-Barr virus (EBV) (Henle et al. 1967). Such cell lines contain virus DNA in addition to cellular DNA (zur Hausen & Schulte-Holthausen, 1970), but the virus genome is present in a repressed state (Hampar et al. 1972). Some cell lines of this type can be superinfected with EBV. This results in an abortive infection, with the expression of several early virus functions (Henle et al. 1970; Gergely, Klein & Ernberg 1971) but no production of virus particles. In the present work, biologically active EBV, labelled with [³H]-thymidine, was partly purified from the culture medium of the cell line P3HR-1 which produces virus (Hinuma et al. 1967). The fate of the radioactive virus DNA was then investigated after abortive infection of two different human lymphoid cell lines.

^* Present address: Biegenstrasse 26, 355 Marburg/L., Germany.

Received 3 February 1972; accepted 7 June 1972.