J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 17 (1972), 343-347; DOI 10.1099/0022-1317-17-3-343
© 1972 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Correction (v18,pNP)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsumura, T.
Right arrow Articles by Schlesinger, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsumura, T.
Right arrow Articles by Schlesinger, R. W.
Agricola
Right arrow Articles by Matsumura, T.
Right arrow Articles by Schlesinger, R. W.

Effects of Ionic Strength on the Release of Dengue Virus from Vero Cells

T. Matsumura*, V. Stollar and R. W. Schlesinger

College of Medicine and Dentistry of New Jersey Rutgers Medical School Department of Microbiology, New Brunswick, New Jersey 08903, U.S.A.

The ionic strength and ionic composition of the medium can markedly affect certain aspects of virus replication or maturation in the case of some picornaviruses (Wallis & Melnick, 1962; Tolskoya et al. 1966; Fiala & Kenny, 1967; Blough et al. 1969; Agol et al. 1970) and in the case of Sindbis virus (SV), a group A togavirus (Waite & Pfefferkorn, 1970). In general, a lowered ionic strength appears to inhibit while a higher ionic strength increases virus yield. Blough et al. also reported enhanced crystallization of rhinovirus in HeLa cells after increasing the concentration of MgCl2 in the medium. Our observations described below show that the yield of dengue virus (DV), a group B togavirus, is also affected by the ionic strength of the medium. However, no clear-cut effect on the crystallization of DV in Vero cells (Matsumura, Stollar & Schlesinger, 1971) was noted after increasing the MgCl2 concentration.

* Present address: Department of Microbiology, Kobe University School of Medicine, Kusunoki-Cho Ikuta-Ku, Kobe, Japan.

Received 15 May 1972; accepted 6 September 1972.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1972 by the Society for General Microbiology.