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Changes in the Constitutive Enzymes of Chick Cells Following Infection with Semliki Forest Virus

Division of Biological Sciences, Warwick University, Coventry CV4 7AL, England

Selective changes were found in the constitutive enzymes of the host cell following infection of chick embryo fibroblast cells with Semliki Forest virus. The changes were limited to soluble dehydrogenases involved in pathways of glucose catabolism. Changes were also found in nucleotide levels which, along with the enzyme changes, suggested that glucose catabolism was stimulated in the virus infected cells. The enzyme changes were prevented by pretreatment of the cells with actinomycin D.

Received 4 September 1972; accepted 28 September 1972.