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Virus Antigens on the Surface of Infected Cells: Binding and Elution of [¹²⁵I]-labelled Antivirus Antibody

Virology Section Laboratory of Microbiology and Immunology National Institute of Dental Research National Institutes of Health, Bethesda, Maryland 20014, U.S.A.

We showed recently that the specific binding of [¹²⁵I]-labelled antivaccinia IgG to the surface of vaccinia-infected cells could be used to detect vaccinia antigens and measure antivaccinia antibody (Hayashi, Rosenthal & Notkins, 1972). The present study shows that this technique can be applied to other viruses and that purified antivirus antibody can be obtained by eluting the specifically-bound immunoglobulins from the surface of infected cells.

Primary rabbit kidney (PRK) cells were prepared as described previously (Hampar et al. 1968). Herpes simplex virus (HSV), type 1, and vaccinia virus, strain CV1–79, were propagated and assayed in PRK cells (Hampar et al. 1968; Kempe et al. 1968). Influenza virus, strain PR-8, was grown in 10-day-old embryonated hen's eggs and assayed by haemagglutination (Davenport & Minuse, 1964). Hyperimmune antisera to each virus were prepared in rabbits (Brier et al. 1971). The gamma globulins were obtained by ammonium sulphate precipitation and fractionated on Sephadex G-200 (Hampar et al. 1968).

Received 10 July 1972; accepted 20 October 1972.