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J Gen Virol 18 (1973), 215-222; DOI 10.1099/0022-1317-18-3-215
© 1973 Society for General Microbiology
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Inactivation and Mutagenesis of Tobacco Rattle Virus by Nitrous Acid

D. J. Robinson

Scottish Horticultural Research Institute, Invergowrie, Dundee, Scotland

The rate of inactivation of tobacco rattle virus RNA by 0.2 M-nitrous acid at pH 4.0 was comparable to that of tobacco mosaic virus RNA. In 0.0025 M-nitrous acid at pH 6.0 this rate was greatly diminished, and was slower than that of intact virus. Possible reasons for this are discussed. Methods are described for the induction of mutants of tobacco rattle virus by nitrous acid, and for the selection of temperature-sensitive mutants that are not characterized by changes in the coat protein gene.

Received 8 August 1972; accepted 10 October 1972.




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