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Institute of Virology, University of Glasgow, Glasgow G11 5JR, Scotland
Nine 5-bromodeoxyuridine-induced temperature-sensitive (ts) mutants were isolated from stocks of a syncytial plaque type mutant (syn) itself derived from the non-syncytial wild-type (syn+) of the GLASGOW strain 17 of herpes simplex virus type 1. The nine ts mutants have been assigned to eight cistrons on the basis of different complementation tests.
Non-syncytial revertants isolated from the original ts syn mutants were used in reciprocal three-factor crosses of the type: tsX syn x tsY syn+ and tsX syn+ x tsY syn. With recombination data from these crosses a linkage map has been constructed. The provisional map which locates nine cistrons is linear and spans about 25 recombination units.
The regular occurrence of plaques with mixed syn/syn+ morphology in the progeny from crosses is reported.
Received 24 August 1972;
accepted 25 October 1972.
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