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Sindbis Virus Maturation

United States Army Medical Research Institute of Infectious Diseases Fort Detrick Frederick Maryland U.S.A.

Sindbis virus, a group A arbovirus, is composed of a nucleocapsid and a glycolipoprotein membrane (Pfefferkorn & Hunter, 1963a; Strauss et al. 1968; Schelle & Pfefferkorn, 1969). The membrane protein is specified by the virus genome (Pfefferkorn & Clifford, 1964; Strauss et al. 1968; Strauss, Burge & Darnell, 1969) whereas the phospholipids and carbohydrate are derived from the host of origin (Pfefferkorn & Hunter, 1963b; Burge & Strauss, 1969; Strauss, Burge & Darnell, 1970) during maturation by budding through the membranes of cytopathic vacuoles (CPV) or the plasma membranes of the infected cell (Grimley, Berezesky & Friedman, 1968; Hackett et al. 1968; Holmes, Wark & Warburton, 1969; Nakai, Shand & Howatson, 1969). We have studied the maturation of Sindbis virus with the use of ferritin-conjugated antibody.

After exhaustive adsorption with normal chick embryo cells, the globulin fraction precipitated from rabbit anti-Sindbis serum (Keckwick, 1940; Strauss et al. 1960) was conjugated with ferritin (Singer, 1959; Hsu, Rifkind & Zabriski, 1963; Rifkind, Hsu & Morgan, 1964).

^* Present address: Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712, U.S.A.

Received 31 August 1972; accepted 17 November 1972.