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The Protein and Nucleic Acid Components of Elderberry Latent Virus

Scottish Horticultural Research Institute, Invergowrie, Dundee, Scotland

The protein and nucleic acid components of elderberry latent virus (ELV) were of similar size to those of tomato bushy stunt virus (TBSV) when examined by electrophoresis in polyacrylamide gels. Estimates of the mol. wts. of the proteins were 40000 (ELV) and 42000 (TBSV), and the estimates of the mol. wts. of the nucleic acids were 1.55 x 10⁶ (ELV), and 1.8 x 10⁶ (TBSV). Buoyant densities in caesium chloride were 1.363 g/cm³ (ELV) and 1.348 g/cm³ (TBSV). To account for differences in sedimentation coefficient and in buoyant density between ELV and TBSV, and in comparison with the structure of 180 + 12 protein subunits proposed for TBSV, we suggest that the most likely number of protein subunits in particles of ELV is 120.

Received 30 October 1972; accepted 1 January 1973.