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Semliki Forest Virus-Chick Embryo Cell Interactions

Microbiological Research Establishment, Porton Down, Salisbury, Wiltshire, England

There is no correlation between virulence in experimental animals and such physical properties of Semliki Forest virus (SFV) as rates of inactivation or electrophoretic and chromatographic distribution patterns. Changes in the surface properties of the virus can be induced in most strains of SFV by addition of polysaccharide derivatives during virus growth in chick embryo cells; these changes cause a variation in the pattern of infection in both chick embryo cells and mice.

Glucosamine and DEAE-dextran both inhibit a function involved in the release of virus from chick embryo cells. It is suggested that one or more of at least five sites may be used during the ‘budding’ of virus from the cell depending on the strain of SFV.

In sugar-free media, mannosamine inhibits transfer of infective material across the cell membrane; both glucose and mannose relieve this inhibition, but relief by galactose is variable, indicating that more than one mechanism may be used. Transfer of infective material across the cell membrane is not always a corollary of attachment of virus to the cell.

Received 13 December 1972; accepted 25 January 1973.