HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Simons, P. J. Search for Related Content PubMed PubMed Citation Articles by Simons, P. J. Agricola Articles by Simons, P. J.

Properties of Mouse Embryo Cells Infected with Murine Sarcoma Virus and Simian Virus 40 simultaneously in vitro

Department of Microbiology University of Western Australia

Serially subcultivated Balb/c mouse embryo cells pass through a non-dividing or amitotic phase prior to becoming an established, growing cell line (Aaronson & Todaro, 1968; Baker & Simons, 1971). Cells in the amitotic phase are not visibly transformed by murine sarcoma virus-Harvey (MSV-H), although there is a low level of replication of the virus (Baker & Simons, 1971). DNA synthesis and cell division are initiated in the amitotic cells by infection with SV 40 (Baker, Simons & Rankin, 1972). The amitotic cells are particularly susceptible to SV 40, 60% of the cells synthesizing DNA by 48 h after infection (Baker et al. 1972). Moreover, simultaneous infection of amitotic cells with SV 40 and MSV-H results in extensive cellular transformation and MSV replication (Baker et al. 1972). This communication describes some of the properties of cells derived from amitotic cell cultures infected simultaneously with SV 40 and MSV-H.

^* Present address: Tobacco Research Council Laboratories, Otley Road, Harrogate, Yorkshire, England.

Received 30 November 1972; accepted 19 February 1973.