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Interaction of p-Chloromercuribenzoate with Adenoviruses. Inactivation of Haemagglutinins and Degradation of Virions of Types 3, 4 and 7

Department of Research and Development, Wyeth Laboratories Inc., Philadelphia, Pennsylvania, U.S.A.

Interaction of p-chloromercuribenzoate with the haemagglutinins of adenovirus types 3, 4 and 7 resulted in the loss of their ability to agglutinate red cells— a loss which can be either reversible or irreversible. The structural integrity of the haemagglutinins of types 3 and 7 was maintained in the process of their reversible inactivation in 0.2 M-tris buffer, but inactivation was irreversible if they were allowed to react with p-chloromercuribenzoate in 0.1 M-borate buffer (pH 8.0) or if the reversibly inactivated haemagglutinins were transferred into the borate buffer even in the absence of the mercurial compound. Thus the reversibly inactivated haemagglutinins are predisposed to irreversible loss of activity. Haemagglutinin of type 4 was irreversibly inactivated in both buffers. Virus particles of adenovirus types 3, 4 and 7 are degraded by treatment with p-chloromercuribenzoate in the borate buffer.

Received 4 September 1967; accepted 30 September 1967.