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Plaque Formation by Arboviruses

Departmento de Virologia, Instituto Venezolano de Investigaciones Cientificas (IVIC) Apartado 1827, Caracas, Venezuela

Cell lines of baby hamster kidney (BHK 21), green monkey kidney (Vero, MA 134), rhesus monkey kidney (MA 104), and rabbit kidney (MA 111) were found suitable for titration and multiplication of arboviruses. Plaque characteristics of 52 arboviruses and 20 virus strains and titres of some of them in BHK 21 and other cell lines were established, using one type of serum-free standard overlay and standard maintenance medium. However, the addition of cortisol or other compounds was necessary to prolong survival of BHK 21 cell sheets, and induce or improve plaque formation.

Received 30 June 1967; accepted 24 October 1967.