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Production of Infectious RNA and Serum-blocking Antigen by Poliovirus Temperature-sensitive Mutants

Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, Australia

Poliovirus ts mutants were cultivated at a temperature (39.6°) too high for their optimal growth but low enough to permit full growth of the parental ts⁺ strain. Tests for production of infectious RNA and serum-blocking antigen showed a gradient of mutant productivity that corresponded closely with an earlier physiological classification of these mutants. No mutant produced fully wild-type yields of either RNA or antigen. In general, group A mutants produced the least RNA and antigen (some yields being undetectable or < 1% of ts⁺ values) while group D mutants produced the most (up to 88% of ts⁺ RNA and 27.6% of ts⁺ antigen). Mutants of groups B and C produced intermediate yields. Six of the mutants (all that were classified belonging to groups A or B) converted their small yield of RNA and antigen to infective virus with an efficiency not significantly different from that of wild-type virus. The remainder (thirteen mutants, most of those classified belonging to groups C or D) matured either or both their RNA and antigen inefficiently and accordingly exhibited a defect in some stage of maturation or assembly.

^* Present address: Department of Preventive Medicine, School of Medicine, University of Washington, Seattle, Washington. Postdoctoral Research Fellow, United States Public Health Service, 1964–66.

Received 15 September 1967; accepted 31 October 1967.