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Immunofluorescent Studies on the Inhibition of Influenza A and B Viruses in Mammalian Cell Cultures by Amines and Ammonium Compounds^*

University of Sheffield, Virus Research Laboratory, Lodge Moor Hospital, Sheffield 10

A quantitative immunofluorescent cell-counting technique was used to investigate the virustatic effect of aminoadamantane, ammonium acetate and a number of aliphatic amines on the development of influenza virus antigens in BHK-21 cell monolayers. Influenza virus strains A2/SCOTLAND/49/57, A/NWS and B/ENGLAND/939/59 were used at high multiplicities of infection in the tests. Quantification of the activity of the antiviral compounds was provided by the direct estimation of the proportion of infected cells in which the production of influenza virus fluorescent antigens was blocked. Comparable results were obtained for A2/SCOTLAND/49/57 virus using the immunofluorescent technique and the more conventional method of measuring antiviral activity by the reduction in the ability of the virus to multiply in tissue culture in the presence of the test compounds.

^* A short account of this work was presented at the 5th International Congress of Chemotherapy, Vienna, 1967.

Received 14 October 1967; accepted 17 November 1967.