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J Gen Virol 2 (1968), 399-426; DOI 10.1099/0022-1317-2-3-399
© 1968 Society for General Microbiology
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The Largest Rabies-specific Antigen in Extracts of Infected Suckling Mouse Brains

W. Katz, Karin M. Larsson and T. H. Mead

C.S.I.R. and U.C.T. Virus Research Unit, University of Cape Town, Republic of South Africa

Extracts of rabies-infected suckling mouse brains purified by precipitation at pH 4.5, freed from smaller antigens by sedimentation at 161, 180g and digested with RNase, DNase and trypsin show in the ultracentrifuge a component of S20 {approx} 16 to 18 which is lacking in extracts of normal suckling mouse brains similarly treated. The largest rabies soluble antigen (‘outer antigen’: Mead, 1962b) has a sedimentation constant S20 {approx} 16 estimated by the ‘biological’ method of Polson & van Regenmortel (1961). The purified antigen appears to consist of rings or possibly single-turn helices about 100 Å in diameter containing about 0.57 µg. pentose (as ribose) per µg. total nitrogen. The antigen also appears to contain deoxypentose. It is resistant to pancreatic RNase, DNase, trypsin and chymotrypsin, has a density of about 1.34 g./cm3. in CsCl and an electrophoretic mobility about 7/8 that of rabbit serum albumin at pH 8.5.

Preparative density-gradient centrifugation in the analytical rotor of the Model E Spinco centrifuge is described. This allows the method to be applied to smaller particles than can be treated in the S.W. 39 rotor.

Received 3 December 1966; accepted 1 December 1967.




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Copyright © 1968 by the Society for General Microbiology.