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J Gen Virol 2 (1968), 437-441; DOI 10.1099/0022-1317-2-3-437
© 1968 Society for General Microbiology
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The Effect of 5-fluoro-deoxyuridine on the Replication of Viral DNA and Synthesis of Polyoma Capsid Protein

R. A. Consigli, H. C. Minocha* and H. Abo-Ahmed{dagger}

Kansas State University, Division of Biology, Subdivision of Molecular Biology and Genetics, Manhattan, Kansas, 66052, U.S.A.

It has been reported that synthesis of specific viral protein requires replication of viral DNA. Various investigators (Flanagan & Ginsberg, 1962; Kjellén, 1962; Gilead & Ginsberg, 1965) found that the formation of virus specific proteins in adenovirus-infected cells was directly dependent upon the synthesis of viral DNA. The analogue 5-fluoro-2-deoxyuridine (FUdR) completely inhibited synthesis of viral antigen in human foetal muscle cells infected with cytomegalovirus (Goodheart, Filbert & McAllister, 1963) and suppressed the production of specific virus antigen in SV40 infected monkey kidney cells (Melnick, Stinebaugh & Rapp, 1964; Gilden et al. 1965). In contrast, the work on pseudorabies virus (Reissig & Kaplan, 1962) suggested that specific viral protein formation can proceed in the absence of apparent viral DNA replication and synthesis of viral protein under conditions of FUdR inhibition has been reported for vaccinia virus (Shatkin, 1963; Salzman, Shatkin & Sebring, 1963; Loh & Payne, 1965) and polyoma virus (Sheinin, 1964).

* Present address; Department of Microbiology, North Carolina State University, Raleigh.

{dagger} Present address; Department of Pediatrics, University of Kansas Medical Center, Kansas City.

Received 18 October 1967; accepted 15 November 1967.




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Copyright © 1968 by the Society for General Microbiology.