J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 2 (1968), 455-459; DOI 10.1099/0022-1317-2-3-455
© 1968 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fox, S. M.
Right arrow Articles by Sonnabend, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fox, S. M.
Right arrow Articles by Sonnabend, J. A.
Agricola
Right arrow Articles by Fox, S. M.
Right arrow Articles by Sonnabend, J. A.

The Use of Batch-type Zonal Ultracentrifuge Rotors for the Isolation and Purification of Viruses

Sylvia M. Fox, G. D. Birnie and D. R. Harvey

Division of Experimental Biology and Virology Imperial Cancer Research Fund, Burtonhole Lane, Mill Hill London, N.W. 7

E. M. Martin and J. A. Sonnabend

National Institute for Medical Research, Mill Hill London, N.W. 7

Continuous-flow zonal ultracentrifugation is now established as an invaluable technique for rapidly concentrating, and largely purifying, milligram amounts of infectious virus from very large volumes of tissue culture fluid (Cline, Nunley & Anderson, 1966; Toplin, 1967; Cline et al. 1967; Fox et al. 1967). The quantities of virus thus obtainable are potentially of use for chemical and physical studies of virus nucleic acid and protein or for production of vaccines. However, for many purposes, sufficient virus can be obtained from volumes of tissue culture fluid of the order of 1 l., whereas continuous-flow zonal rotors are most suited for handling volumes of 5 l. and upwards. Moreover, a continuous-flow rotor is a specialized piece of equipment and so is available in relatively few laboratories. Batch-type zonal rotors, on the other hand, are much more widely available since they are used for separating a variety of subcellular components (Anderson et al. 1967).

Received 18 November 1967; accepted 17 January 1968.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1968 by the Society for General Microbiology.