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J Gen Virol 20 (1973), 253-256; DOI 10.1099/0022-1317-20-2-253
© 1973 Society for General Microbiology
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Electrofocusing of Hepatitis B Antigen

C. R. Howard and A. J. Zuckerman

Hepatitis Research Unit Department of Microbiology London School of Hygiene & Tropical Medicine, London, WC1E 7HT

Hepatitis B (Australia) antigen was isolated from normal serum proteins by gel filtration and electrofocusing. The latter technique revealed a heterogeneity of the small spherical particles associated with hepatitis B antigen activity, there being a variance in determined isoelectric points according to the serological subtype studied. Hepatitis Bantigen isolated in this manner was aggregated by concanavalin A.

Received 16 November 1972; accepted 3 May 1973.




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