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J Gen Virol 20 (1973), 267-276; DOI 10.1099/0022-1317-20-3-267
© 1973 Society for General Microbiology
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Isolation of Glucosamine from the Capsids of a Picornavirus

S. Halperen*, H. O. Stone{dagger} and B. D. Korant

Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898, U.S.A.

The purified capsids of echovirus 12 were found to contain radioactivity when radioactive glucosamine was provided for virus-infected cultures. Radioactivity was identified with glucosamine, after recovery from the virus particles by acid hydrolysis and paper chromatography. Although the label was not removable from the isolated virus particles without destruction of virus, polyacrylamide gel electrophoresis showed that it was not covalently bound to any of the four virus polypeptides since it migrated more rapidly and was identical electrophoretically to the glucosamine-labelled fraction isolated from uninfected cells. Glucosamine labelling of virus capsids was only achieved during virus multiplication, since labelled fractions from uninfected cells did not bind to virus capsids. Its apparently low mol. wt., lack of associated virus or cellular proteins, lack of labelling of virus with other radioactive sugars, and the similar electrophoretic migration of free N-acetyl glucosamine suggested that the component is an acetylated product that is trapped during the assembly of the virus.

* Present address: Department of Oral Pathology, Baylor College of Dentistry, 800 Hall Street, Dallas, Texas 75226, U.S.A.

{dagger} Department of Microbiology, University of Kansas, Lawrence, Kansas 66044, U.S.A.

Received 22 August 1972; accepted 6 April 1973.




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Copyright © 1973 by the Society for General Microbiology.