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Parvoviruses

NERC, Unit of Invertebrate Virology, South Parks Road, Oxford, England

J. F. Longworth

DSIR, Entomology Division, Nelson, New Zealand

The parvoviruses provide considerable uinterest because of their small size and possesion of single-strandede linear DNA. Tese viruses appear to have an affirnity for tissue cell lines wbhere they are usually present as inappare nt infections. The require rapidly growing cells for maximum replication to occur. Present knowledge indicates that mammals and insects are the only hosts, but it would be worth while to look at cell systems derived from other vertebrates, e.g. fish and reptiles, and also in the rapidly growing number of invertebrate cell lines. In this connexion the technique of extracting the cell monolayers with an alkaline barate or glycine buffer at pH 9.0 (Hallauer & Kronauer, 1960) could be invaluable. This method allows the liberation of cell-associatred viruses without damage to the extracted cells, and so they can be treated at frequent intervals during the growth cycle. This is particularly important with parvoviruses as they shoe alternating cycles of high and low concentrating of virus (Hallauer & Kronauer, 1962).