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The Structure of Tobacco Rattle Virus Ribonucleic Acids: Common Nucleotide Sequences in the RNA Species

Department of Virology, Medical School, University of Birmingham, B15 2TJ, England

It has been shown by competition hybridization experiments that common sequences of about 600 nucleotides are present in TRV long and short particle RNA species. Heteroduplex molecules have been formed by the hybridization of short particle RNA strands with long complementary strands derived from tissue infected with a ‘defective isolate’ of the virus. The heteroduplex molecules were shown to have a lower melting temperature than either the long-long or short-short homoduplexes, indicating some mismatching of base pairs. The results are discussed in the light of our present knowledge about the replication of viruses.

Theoretical equations have been derived to describe RNA-RNA competition hybridization and duplex formation.

Received 17 April 1973; accepted 8 June 1973.