HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sangar, D. V. Articles by Brown, F. Search for Related Content PubMed PubMed Citation Articles by Sangar, D. V. Articles by Brown, F. Agricola Articles by Sangar, D. V. Articles by Brown, F.

Reaction of Glutaraldehyde with Foot-and-Mouth Disease Virus

Animal Virus Research Institute, Pirbright, Surrey, U.K.

Treatment of foot-and-mouth disease virus with 4% glutaraldehyde increases the diam. of the particles by 25% and makes them permeable to phosphotungstic acid so that they appear empty. The treated particles also resemble naturally-occurring empty particles in their low sedimentation coefficient (about 75S) but, in contrast to empty particles, they have a normal content of RNA and a higher than normal buoyant density in caesium chloride. The RNA can be removed from fixed particles by ribonuclease. Two models are suggested which account for these alterations in the structure of the virus particles. These results show that fixation with glutaraldehyde, far from maintaining the structural integrity of the virus particles, leads to considerable alterations in the arrangement of the RNA and protein subunits.

Received 10 April 1973; accepted 20 June 1973.