HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cohen, J. Articles by Scherrer, R. Search for Related Content PubMed PubMed Citation Articles by Cohen, J. Articles by Scherrer, R. Agricola Articles by Cohen, J. Articles by Scherrer, R.

Physico-chemical and Morphological Features of Infectious Pancreatic Necrosis Virus

Institut National de la Recherche Agronomique, Station de Recherches de Virologie et d'Immunologie, 78850 — Thiverval-Grignon, France

Infectious pancreatic necrosis (IPN) virus has been purified and some physicochemical properties of its nucleic acid and protein moieties have been determined. The buoyant density of the virus particle in CsCl is 1.35 g/ml; degraded particle banded at a density of 1.31 g/ml. The virus nucleic acid was found to be double-stranded, according to several different criteria, namely resistance to pancreatic RNase, low density in Cs₂SO₄ (1.615), sharp melting curve with a T_m of 89 °C in hypotonic buffer, and base composition. In a sucrose gradient the bulk of the virus RNA banded as a single peak (14 S); a small part of the virus nucleic acid was found at the top of the gradient. By gel electrophoresis virus RNA was resolved into three peaks corresponding to double-stranded molecules with mol. wt. ranging from 2.85 x 10⁶ to 2.55 x 10⁶. Gel analysis of virus proteins revealed three polypeptides of mol. wt. 80, 50 and 30 x 10³, representing respectively 3%, 68% and 29% of the total protein. On the basis of these results IPN virus appears to be related to some reo-like viruses, having a single protein shell and a single class of double-stranded RNA.

Received 22 May 1973; accepted 26 July 1973.

This article has been cited by other articles:

				R. A. Villanueva, J. L. Galaz, J. A. Valdes, M. M. Jashes, and A. M. Sandino Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus J. Virol., December 15, 2004; 78(24): 13829 - 13838. [Abstract] [Full Text] [PDF]