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J Gen Virol 21 (1973), 507-513; DOI 10.1099/0022-1317-21-3-507
© 1973 Society for General Microbiology

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A Salt-stable Mutant of Cowpea Chlorotic Mottle Virus

J. B. Bancroft*, M. W. Rees, M. W. Johnson and J. R. O. Dawson

John Innes Institute, Colney Lane, Norwich, NOR 70F, England

The properties of a mutant of cowpea chlorotic mottle virus which, although infective, cannot be disassembled in 1 M-NaCl, pH 7.5, are described. The mutant coat protein contains a lysyl to arginyl replacement which affects the hydrodynamic and titration characteristics of the virus which are discussed in regard to the anomalous ionization of a carboxyl-carboxylate pair and a lysyl residue.

* Present address: Department of Plant Sciences, University of Western Ontario, London, Ontario, Canada.

Received 19 June 1973; accepted 30 July 1973.





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