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J Gen Virol 21 (1973), 515-521; DOI 10.1099/0022-1317-21-3-515
© 1973 Society for General Microbiology
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The Replication of Polyoma DNA

L. V. Crawford, Christine Syrett and Annelie Wilde

Imperial Cancer Research Fund Laboratories, P.O. Box 123, Lincoln's Inn Fields, London, WC2A 3PX

Measurement of replicating molecules of polyoma virus DNA after digestion with endonuclease R1 shows that DNA replication is bidirectional, starting predominantly at a specific site. In both large plaque and small plaque polyoma virus DNA this site is 29 ± 2% of the total length of the DNA from the cleavage site of the endonuclease R1.

Received 28 June 1973; accepted 31 July 1973.




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Copyright © 1973 by the Society for General Microbiology.