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Induction of Virus Ribonucleic Acids in Non-permissive MSV-IF + Cells Infected with NDV

Unité de Recherches sur les Infections virales, Institut National de la Santé et de la Recherche Médicale, U. 43, Hôpital St. Vincent de Paul, 74, avenue Denfert Rochereau, 75014 Paris, France

J. Huppert

Institut Gustave Roussy, Groupe de Recherches No. 8 du CNRS, Villejuif (94), France

Mouse cells (MSV-IF +) are completely non-permissive to NDV; however, they produce interferon when NDV is used as an inducer. Eight h after infection these cells synthesize a virus ‘minus’ strand RNA which anneals to the extent of 57 to 97% with virus ‘plus’ strand RNA. This synthesis disappears gradually up to 48 h after infection, and is not modified by the incubation temperature (37 °C or 40 °C) of the infected cells.

Received 2 May 1973; accepted 27 July 1973.